3种方法检测128例儿童肺炎支原体感染结果分析

摘要

Objective:To compare the results of three methods for the detection of mycoplasma pneumoniae (MP) infection. Methods:128 cases of children with respiratory tract infection were treated with MP throat swab culture,serum enzyme linked immunosorbent assay (ELISA) and sputum MP-DNA-PCR test.Results:8 cases had positive MP detected by MP-DNA-PCR (6.25%).64 cases had positive MP detected by (IgM) serum enzyme linked immunosorbent assay (ELISA) (50.2%).25 cases had positive MP detected by rapid culture of MP throat swab (24.35%).There were statistically significant differences of positive rate of serum MP-IgM among all age groups (P<0.05).Conclusion:Combined the rapid culture of MP throat swab,serum enzyme linked immunosorbent assay (ELISA) and sputum MP-DNA-PCR detection could improve the detection rate of MP infection.%目的：比较3种检测方法检查肺炎支原体(MP)感染的结果。方法：收治呼吸道感染患儿128例，均给予MP咽拭子快速培养、血清酶联免疫吸附试验(ELISA)法检测和痰 MP-DNA-PCR 检测。结果：MP-DNA-PCR 法检出MP阳性8例(6.25%)。IgM ELISA法检出MP阳性64例(50.2%)。MP咽拭子快速培养测检出MP阳性25例(24.35%)。各年龄组之间血清MP-IgM检测法的阳性率比较，差异有统计学意义(P＜0.05)。结论：联合采用MP咽拭子快速培养、ELISA法检测和痰MP-DNA-PCR检测可以提高MP感染的检出率。