目的 探讨趋化因子受体-2 (CCR2)基因的单核苷酸多态性(SNP)位点V64I(CCR2-V64I)与宫颈癌及宫颈上皮内瘤变(CIN)的相关性.方法 采用聚合酶链式反应-序列特异性引物(PCR-SSP)、琼脂糖凝胶电泳及基因测序联合检测分析方法,对40例原发性宫颈癌、45例CIN和60例同期健康体检者进行基因多态性的检测.结果 基因测序与琼脂糖凝胶电泳对CCR2-V64I的检测结果一致.CCR2-V64I G/G、G/A和A/A基因型频率在宫颈癌中分别为27.5％、12.5％和60.0％,在CIN中分别为53.3％、24.4％和22.2％,而在对照组中分别为38.3％、31.7％和42.9％,宫颈癌组与对照组的基因型分布差异有统计学意义(P＜0.01),但CIN组与对照组之间差异无统计学意义(P＞0.05).携带A等位基因可以增加宫颈癌的发病风险(OR =2.32,95％ CI:1.29～4.17),但并不增加CIN的发病风险(OR=0.62,95％ CI:0.35 ～ 1.09).结论 宫颈癌及CIN中均存在CCR2-V64I基因多态性,但其与CIN无关,而与宫颈癌患病风险有关,表明CCR2-V64I基因多态性在宫颈癌的发生、发展中可能具有重要意义.%Objective To investigate the relationship between the gene single-nucleotide polymorphisms (SNP) site of V64I within chemokine receptor 2 (CCR-2) and cervical cancer and cervical intraepithelial neoplasia (CIN).Methods Polymerase chain reaction sequence specific primers (PCR-SSP),electrophoresis and gene sequencing analysis were applied respectively to detect the CCR2-V64I gene SNP in 40 patients with primary cervical cancer,45 patients with CIN and 60 health controls.Results Gene sequencing analysis and electrophoresis showed the same results on the CCR2-V64I gene SNP.The frequencies of G/G,G/A and A/A genotype of V64I polymorphism within CCR2 gene were 27.5％,12.5％ and 60.0％ in cervical cancer,53.3％,24.4％ and 22.2％ in CIN,and 38.3％,31.7％ and 42.9％ in control group.There was significant different in the distribution of polymorphism of CCR2V641 gene polymorphism between cervical cancer and normal control(P ＜ 0.01),but there was no significant difference between CIN and the normal control(P ＞ 0.05).Harboring allele A increased the risk of cervical cancer (OR =2.32,95％ CI:1.29-4.17),but did't increase the risk of CIN (OR =0.62,95％ CI:0.35-1.09).Conclusion CCR2 gene polymorphism exists in cervical cancer and CIN,which correlates with the risk of cervical cancer but not with CIN,indicating that CCR2-V641 gene polymorphism may play an important role in the occurrence and development of cervical cancer.
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