肿瘤干细胞相关基因Bmi-1和POU5F1在子宫颈癌中的表达及临床意义

摘要

目的 探讨肿瘤干细胞相关基因Bmi-1、POU5F1在子宫颈癌组织中的表达及其与临床病理特征的关系.方法 采用实时荧光定量PCR(QRT-PCR)检测69例子宫颈癌组织和16例子宫颈癌组织及其配对的癌旁新鲜组织标本中Bmi-1 mRNA、POU5F1 mRNA的表达水平.结果 子宫颈癌组织中Bmi-1 mRNA、POU5F1 mRNA相对表达量分别为1.51±0.05和1.75±0.17,高于配对癌旁组织的1.16±0.17和1.46±0.18,差异均有统计学意义(P＜0.05).Bmi-1 mRNA、POU5F1 mRNA在宫颈癌组织与配对癌旁组织中表达的倍比关系分别为1.31±0.49和1.20±0.95.Bmi-1 mRNA表达与民族、淋巴结转移、临床分期及组织学分级有关(P＜0.05),而与年龄、肿瘤大小及病理类型无关(P＞0.05).POU5F1 mRNA表达与民族、肿瘤大小、淋巴结转移、临床分期及组织学分级有关(P＜0.05),而与年龄及病理类型无关(P＞0.05).结论 肿瘤干细胞相关基因Bmi-1、POU5F1在子宫颈癌组织中高表达,两者可能与子宫颈癌的不良生物学特性关系密切,可作为评估子宫颈癌生物学行为的潜在分子标志物.%Objective To explore the expression of cancer stem cell related genes Bmi-1,POU5F1 in cervical cancer and their relationship with clinicopathological chracteristics.Methods The expression of Bmi-1,POU5F1 at mRNA level in 69 cervical cancer tissues and 16 cervical cancer tissues plus their para-carcinoma tissues were detected by real-time fluorescent quantitative PCR (QRT-PCR).Results The expression levels of Bmi-1 mRNA,POU5F1 mRNA in cervical cancer tissues were 1.51±0.05 and 1.75± 0.17,higher than 1.16±0.17 and 1.46±0.18 of their counterparts of the para-carcinoma tissues with statistically significant (P＜ 0.05).The relative ratio of level of Bmi-1 mRNA,POU5F1 mRNA was 1.31±0.49 and 1.20±0.95 between cervical cancer tissues and their para-carcinoma tissues.The expression of Bmi-1 mRNA was related with ethnic,lymph node metastasis,clinical staging and histological grade (P＜0.05),but not with age,tumor size and pathological type (P＞0.05).The expression of POU5F1 mRNA was related with ethnic,tumor size,lymph node metastasis,clinical staging and histological grade (P＜0.05),but not with age and pathological type (P＞0.05).Conclusion There was a relative high expression of Bmi-1,POU5F1 in cervical cancer.Bmi-1,POU5F1 expression may be associated with adverse biological characteristics of cervical cancer,and may seive as a potential indicator for molecular markers of cervical cancer biological behavior.