RECOMBINANT VECTOR PROMOTING POU5F1 PROTEIN OVEREXPRESSION AND CONSTRUCTION METHOD THEREFOR

摘要

Provided in the present inventon is a recombinant vector for promoting the overexpression of the POU5F1 protein, belonging to the technical field of biology. The recombinant vector comprises a POU5F1 coding sequence and a pEGFP-C1 vector. The pEGFP-C1 vector comprises XhoI and EcoRI restriction sites, and the POU5F1 sequence is forwardly inserted into the restriction sites. The recombinant vector for promoting the overexpression of the POU5F1 protein provided by the present invention has the advantages of a high transfection efficiency, promoting the overexpression of a BNP protein, and consistent and stable expression, and the construction method therefor is also simple.