Objective; To investigate the relationship between angiontensin II type 2 receptor (AT2R) gene A1675G polymorphism and familial hypertension.Methods: A pedigree based case-control study was conducted in two groups. The subjects with hypertension family history ( HF+) group, that including 30 pedigrees with 120 subjects, and he subjects without hypertension family history (HF-) group, that including 29 pedigrees with 115 subjects. AT2R gene A1675G polymorphism was detected by PCR, and AT2R protein expression was examined by ELBA.Results; HF+ group had the increased AA, AG genotype frequencies than those in HF- group (53.3% , 16.7%) vs. (38. 2% ,12.2% ) .decreased GG frequencies (30. 0% vs.49.6% ), increased allele frequencies of A (61.7% vs. 44. 3% ) , decreased allele frequencies of G(38. 3% vs. 55.1% ) ,P<0.05 respectively. The AT2R protein expression in the AG genotype and GG genotype of HF+ group was lower than that in HF-group,(43.42± 12.4) ng/ml vs. (52. 35±11.6) ng/ml and(53. 23± 15.3)ng/ml vs. (65. 9±16.9) ng/ml,P<0.05 respectively.Conclusion: A1675G polymorphism in AT3R gene is obvious related to subjects with hypertension family history. G allele frequency and AT2R have the protective role in familial hypertension development.%目的:探讨血管紧张素Ⅱ-2型受体(AT2R)A1675G基因多态性与家族性高血压的相关性.方法:选取高血压家系(高血压家系组)30个(120例受试者)和正常血压家系(正常血压家系组)29个(115例受试者)进行对照研究.采用聚合酶链式反应(PCR)技术分析AT2R A1675G基因多态性；采用酶联免疫吸附试验(ELISA)技术测定AT2R的表达.结果:高血压家系组AA型、AG型频率分布(53.3％、16.7％)比正常血压家系组(38.2％、12.2％)均升高,GG型频率分布(30.0％ vs 49.6％)降低,高血压家系组比正常血压家系组等位基因A的频率分布(61.7％vs44.3％)升高、G的频率分布(38.3％vs 55.7％)降低,差异均有统计学意义(P＜0.05).高血压家系组与正常血压家系组比较,AG型患者和GG型患者的AT2R表达浓度[(43.4±12.4) ng/ml vs(52.4±11.6) ng/ml,(53.2±15.3) ng/ml vs (65.9±16.9)ng/ml]均降低,差异有统计学意义(P＜0.05). 结论:AT2R基因A1675G多态性与家族性高血压有明显相关性；G基因和AT2R在家族性高血压的发病中起着重要的保护作用.
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