芥蓝花薹中莱菔硫烷含量的 HPLC 分析

摘要

以23份芥蓝纯合自交系和21份杂交 F1为试材，采用反相高效液相色谱法（RP-HPLC）测定花薹中莱菔硫烷含量，分析芥蓝花薹中莱菔硫烷含量的变化规律。结果表明：44份芥蓝材料花薹中莱菔硫烷含量变异范围为46.89～419.45 mg·kg-1（DW），供试材料间莱菔硫烷含量差异达显著水平。获得了1份莱菔硫烷含量较高的 F1材料 A86，含量为419.45 mg·kg-1（DW）；3份莱菔硫烷含量较高的高代纯合自交系材料 A1、A17和 A53，含量分别为298.50、354.19、361.06 mg·kg-1（DW），可用于芥蓝抗癌新品种的选育和利用。%Forty four lines of Chinese kale(Brassica albograbra L.H.Bailey)including 23 high inbred lines and 21 F1 generations were used to detect the sulforaphane contents in flower stalk of Chinese kale by RP-HPLC,and to analyze the changing rule in sulforaphane contents.The results showed that the changing range of sulforaphane contents in 44 Chinese kale was 46.89-419.45 mg·kg-1(DW),and there were significant differences between sulforaphane contents of the testing lines.Four lines were detected with higher sulforaphane contents,among them one line was F1 generation A86 with 419.45 mg·kg-1(DW)sulforaphane content,the other 3 were inbred lines A1,A17 and A53.Their sulforaphane contents were 298.50 mg·kg-1(DW),354.19 mg·kg-1(DW),361.06 mg·kg-1(DW),respectively.They could be used for breeding of Chinese kale in anti-cancer and marketing promotion.