利用辣味辣椒Perennial（Capsicum annuum）和无辣味辣椒83-58种内重组自交系群体构建的辣椒种内遗传图谱，以温室和露地栽培条件下无辣味辣椒77013A与重组自交系群体各株系杂交F1果实的辣椒素和二氢辣椒素含量作为表型性状进行分析。结果表明，温室和露地栽培条件下辣椒素和二氢辣椒素的含量和比值差异明显。对露地和温室栽培条件下辣椒果实辣椒素和二氢辣椒素含量、总和及其比值进行了QTL定位，共获得16个关于辣椒素和二氢辣椒素含量的QTL位点，分布在辣椒第2、4、12号染色体上，2号染色体上同时控制辣椒素、二氢辣椒素、二者比值和辣椒素总量的主效QTL位点cap2.1、dhp2.1、C/D2.1和（C+D）2.1，均在露地和温室被检测到，其LOD值大于5.0，贡献率为20.2%～76.6%，侧翼标记均为BD76366和Pun1，12号染色体上调控辣椒素总量的微效QTL也在温室和露地同时被检测到，其他QTL位点未在两种环境下同时定位到。%In the present study,we constructed an intraspecific genetic map by using a recombinant inbred lines crossed by pungent pepper ‘Perennial’(Capsicum annuum) and non-pungent pepper ‘83-58’,and analyzed capsaicin and dihydrocapsaicin contents investigated on a test cross progeny by non-pungent pepper ‘77013A ’and RILs (‘83-58’בPerennial’) both in greenhouse and open fields. And the differences in capsaicin content,dihydrocapsaicin content,total capsaicinoids and capsaicin/dihydrocapsaicin ratio between greenhouse and open fields were obvious. We did QTL mapping on them and found 16 QTLs located in chromosome 2,4 and 12. The major QTLcap2.1,dhp2.1,C/D2.1and (C+D)2.1 on chromosome 2 that controlling capsaicin content,dihydrocapsaicin content,total capsaicinoids content and capsaicin/dihydrocapsaicin ratio, was detected in both greenhouse and open fields. The LOD was above 5.0,accounting for 20.2%-76.6% of the phenotypic variation. The interval markers were BD76366 and Pun1. We also found minor QTLs in P12 that regulating total capsaicinoids content in 2 environments. Other minor QTLs were not detected at the same time in these 2 environments.
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