番茄黄化曲叶病毒抗性检测DNA提取方法的优化及应用

摘要

通过对传统DNA提取方法进行优化和改进,得到一种快速提取番茄DNA的方法.综合比较改进法、商品化试剂盒(经典法)和其他试剂盒(快速法)提取番茄DNA的效果.结果表明:改进法相比经典法提取的DNA质量稍有差别,但用时较少,完全可以满足后续PCR试验要求,尤其适用于番茄黄化曲叶病毒的大规模抗性检测;同时该法无液氮及其他有毒溶剂的加入,不仅节省了试验时间和试验成本,而且大大降低了试验的危险性.%The rapid and efficient method for tomato DNA extraction has been optimized and improved based on the conventional DNA extraction methods.The comparation improved method,the classic method (commercial kits) and rapid method (other kits) was carried out by the PCR analysis.The results showed that DNA obtained by improved method was slightly different comparing to the classic method with less time and funds when faced with large amount of test samples for detecting Tomato yellow leaf curl virus TYLCV) resistance without the use of liquid nitrogen and other toxic solvents throughout the experiment.