马铃薯早疫病拮抗菌的筛选及其代谢产物的抑菌活性

摘要

Taking Alternaria solani as a target,this study screened the biocontrol strain BAF-6 with strong antagonism against pathogenic bacteria of potato early blight by plate confrontation method. The strain was identified as Bacillus amyloliquefaciens through morphological,physiological and biochemical tests and 16S rDNA sequence analysis. The extracellular metabolites of the strain after 60-80 ℃ heat treatment,its antibacterial activity was over 95%. Under UV irradiation for 8 hours,or when the pH value was 2-10,its antibacterial properties were still stable. After protease treatment the strain antibacterial activity was still over 50%.%以马铃薯早疫病病原菌茄链格孢菌(Alternaria solani)为指示菌,通过平板对峙法,筛选出菌株及其发酵滤液对马铃薯早疫病病原菌均具有较强拮抗作用的生防菌株BAF-6;通过形态特征、生理生化特性鉴定以及16S rDNA序列分析,该菌株为解淀粉芽孢杆菌(Bacillus amyloliquefaciens).菌株发酵滤液经60~80℃热处理,抑菌活性均在95%以上;紫外线照射8 h或在pH值为2~10的酸碱环境下,发酵滤液的抑菌活性稳定;经蛋白酶处理后抑菌活性仍达50%以上.