速绫止血纱布结合封闭式负压引流对创面肉芽组织生成及愈合的影响

摘要

Objective To study effect of surgical hemostatic gauze in combination with vacuum-assisted closure on wound healing and granulation tissue of experimental animals. Methods Eighty healthy SD mouses were randomized into four groups:model group, surgical hemostatic gauze group,vacuum-assisted closure group, and sugical hemostatic gauze plus vacuum-assisted closure group(Abbreviations:composite group). Each group had twenty animals. Removal of skin on the back of each SD mouses were made into a wound model. The wounds of model group healed naturally, the wounds of the remaining groups were treated with surgical hemostatic gauze, vacuum-assisted closure, and surgical hemostatic gauze plus vacuum-assisted closure, respectively. Ten wounds of each group were randomized into four patches. On days 4, 8 and 12, the former three patches were used for observing the granulation tissue formation rate, while the fourth patch for observing the healing time. The granulation tissue formation rate, healing time. Results On day 4, there were no significant differences in the healing rate among different groups (P>0.05). The healing speed of the vacuum-assisted closure group and the surgical hemostatic gauze plus vacuum-assisted closure group accelerated rapidly after four days. On day 8, the healing rate ranged from high to low, as surgical hemostatic gauze plus vacuum-assisted closure group, vacuum-assisted closure group, surgical hemostatic gauze group, and model group. The differences among different groups were significant (P<0.05). Each group healing time from fast to slow sort ibid. Conclusion Surgical hemostatic gauze in combination with vacuum-assisted closure can obviously promote wound healing.%目的：观察速绫止血纱布和封闭负压引流技术相结合对实验动物创面肉芽组织生成及愈合的影响。方法选用80只健康、清洁SD大鼠随机分为对照组、速绫止血纱布组、封闭负压引流组、速绫止血纱布复合封闭负压引流组(简称复合组)，每组20只。于每只大鼠背部切除皮肤制作成创面模型。模型组创面自然愈合，速绫止血纱布组以两层速绫止血纱布组敷于创面上；封闭负压引流组给予创面封闭负压引流治疗。速绫止血纱布复合封闭负压引流组：两层速绫止血纱布组敷于创面上，后覆盖封闭负压引流治疗。每组20只动物随机分3批各20个创面，第1、2、3个批次分别用于4、8、12 d取材观察创面的肉芽生成率。第4个批次观察创面愈合时间。主要观察：①创面的肉芽生成率；②创面的愈合时间。结果①各组创面第4天愈合率比较差异无统计学意义(P>0.05)。4 d以后复合组及封闭负压引流组愈合速度明显加快。第8天愈合率由高到低依次为复合组、封闭负压引流组、速绫止血纱布组、模型组，组间比较差异有统计学意义(P<0.05)。各组愈合时间由快到慢排序同上。结论速绫止血纱布复合封闭负压引流技术可以更好地促进创面愈合。