玫瑰黄链霉菌Men-myco-93-63胞外分泌蛋白对黄瓜白粉病的诱导抗性初报

摘要

To explore induced resistance of Strept omyces roseoflavus Men-myco-93-63, extracellular secretary proteins were isolated from non-organism fermentation supernatant using ammonium sulfate precipitation and then subjected to SDS-PAGE electrophoresis with molecular weights of 15-60 kD. The results showed that the protein had no inhibitive effect on 8 plant pathogens while could induce hypersensitive reaction in tobacco leaves with watery lesions and necrotic spot appearing 12 h and 24 h after treatment. Indoor pot tests showed the protein could induce cucumber resistance to powdery mildew with controlling efficiency of 54.49%.%为了明确玫瑰黄链霉菌Men-myco-93-63胞外分泌蛋白是否可诱导对黄瓜白粉病的抗病性,通过硫酸铵沉淀的方法,从Men-myco-93-63的发酵液中分离出蛋白质粗提物,经SDS-PAGE电泳检测,蛋白质粗提物相对分子量在15~60 kD范围内.该蛋白质粗提物对8种供试植物病原菌均未表现出抑制作用.但能诱导烟草的过敏反应,处理12 h后,处理部位出现水渍状,24 h后出现坏死斑.室内盆栽试验表明,该蛋白质粗提物可以诱导黄瓜对白粉病的抗性,诱抗效果为54.49%.