OBJECTIVE: To compare the effects of Euphorbia ebracteolata vs. Euphorbia fischeriana on the proliferation of human hepatoma carcinoma cell BEL-7402. METHODS: Rats were given E. Ebracteolata^.097,0.485,0.242 5 g-kg"1) and E. Fischeriana (0.05,0.025,0.0125 g-kg~')intragastrically once a day for consecutive 3 days. Blood sample was collected from abdominal .. Aorta of rats after the last administration. Human hepatoma carcinoma cell BEL-7402 was cultured in isolated serum and nutrient solution in vitro. The growth inhibition rate was determined by MTT colorimetric assay and colony-forming test was used for the determination of formation rate of clone. RESULTS: E. Fischeriana inhibited the proliferation of BEL-7402 and the colone formation rate significantly and its effect was positively correlated with drug dosage. E. Ebracteolata had no significant effect on the proliferation of BEL-7402 and the colone formation rate. CONCLUSION: E. Fischeriana has significant inhibition effect on the proliferation of human hepatoma carcinoma cell BEL-7402 and it is superior to E. Ebracteolata.%目的:对比研究月腺大戟与狼毒大戟对人肝癌细胞BEL-7402体外增殖的影响.方法:灌胃月腺大戟(0.097、0.485、0.2425 g·kg-1)、狼毒大戟(0.05、0.025、0.0125 g·kg-1)混悬液,每天1次,连续3d,末次给药后自大鼠腹主动脉采血,以分离的血清加培养液体外培养人肝癌细胞BEL-7402；MTT比色法测定生长抑制率；通过集落形成试验测定克隆形成率.结果:狼毒大戟对BEL-7402细胞增殖和克隆形成率有明显的抑制或降低作用,且与给药剂量呈正相关；月腺大戟对BEL-7402细胞增殖和克隆形成率无明显影响.结论:狼毒大戟对人肝癌细胞BEL-7402体外增殖抑制作用明显强于月腺大戟.
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