HPLC法同时测定仙灵骨葆胶囊中二氢丹参酮Ⅰ、隐丹参酮、丹参酮Ⅰ和丹参酮ⅡA的含量

摘要

OBJECTIVE:To establish a method for simultaneous determination of dihydrotanshinone Ⅰ,cryptotanshinone,tanshinone Ⅰ and tanshinone ⅡA in Xianling gubao capsules.METHODS:HPLC method was adopted.The determination was performed on UltimateTM XB-C18 column with mobile phase consisted of 0.1％ formic acid-acetonitrile (gradient elution) at the flow rate of 1.0 ml/min.The detection wavelength was set at 270 nm,and sample size was 10 μl.The column temperature was 30 ℃.RESULTS:The linear ranges of dihydrotanshinone Ⅰ,cryptotanshinone,tanshinone Ⅰ,tanshinone Ⅱ A were 0.0532-5.32 μg/ml (r=0.9995),0.0512-5.12 μg/ml (r=0.9999),0.0508-5.08 μg/ml (r=0.9996),0.0537-5.37 μg/ml (r=0.9997),respectively.The average recoveries of four active ingredients were 97.93％ (RSD=0.72％,n=6),99.66％ (RSD=0.38％,n=6),99.02％ (RSD=0.54％,n=6) and 99.92％ (RSD=0.22％,n=6),respectively.RSDs of precision,stability and reproducibility tests were all lower than 1.42 ％.CONCLUSIONS:The method is rapid,accurate and repeatable,and can be used for quantitative determination of 4 liposoluble constituents in Xianling gubao capsules.%目的:建立同时测定仙灵骨葆胶囊中二氢丹参酮Ⅰ、隐丹参酮、丹参酮Ⅰ和丹参酮ⅡA含量的方法.方法:采用高效液相色谱法.色谱柱为UltimateTM XB-C18柱,流动相为0.1％甲酸-乙腈(梯度洗脱),流速为1.0 ml/min,检测波长为270 nm,进样量为10 μl,柱温为30℃.结果:二氢丹参酮Ⅰ、隐丹参酮、丹参酮Ⅰ、丹参酮ⅡA检测质量浓度分别在0.0532～5.32、0.0512～5.12、0.0508～5.08、0.053 7～5.37 μg/ml范围内与峰面积积分值呈良好的线性关系(相关系数分别为0.9995、0.9999、0.9996、0.9997);精密度、稳定性、重复性试验的RSD均≤1.42％;平均加样回收率分别为97.93％、99.66％、99.02％、99.92％,RSD分别为0.72％、0.38％、0.54％、0.22％(n=6).结论:该方法快速、准确、重复性好,可同时定量仙灵骨葆胶囊中4种丹参脂溶性成分.