红毛鸡干燥体醇提物对血虚模型小鼠的补血作用研究

摘要

OBJECTIVE:To study blood enriching effect of Centropus sinensis dried body alcohol extract on the blood-deficien-cy model mice. METHODS:60 mice were randomly divided into normal group,model group,positive control group(Compound E’jiaojiang,0.1 ml/10 g)and C. sinensis dried body alcohol extract low-dose,medium-dose and high-dose groups(2.5,5.0,10.0 g/kg),with 10 mice in each group. Except for normal group,other groups were hypodermically injected with APH saline solution on 2nd day and 5th day,and then intraperitoneally injected with CTX saline solution from 6th day to 9th day respectively,which was to establish the blood-deficiency model. From the 1st day,the mice in normal group and model group were intragastrically giv-en constant volume of distilled water,and treatment groups were given relevant medicine intragastrically,once a day,for consecu-tive 9 days. At the end of treatment,WBC,RBC,HGB and HCT were detected by automatic hematological analysis;the thymus index,spleen index,liver index were counted;and the activities of Na+-K+-ATPase,Ca2+-Mg2+-ATPase of red cell membrane and T-AOC and SOD in liver were determined with kits respectively. RESULTS:Compared with normal group,WBC,RBC,HGB, HCT,thymus index,the activities of Na+-K+-ATPase,Ca2+-Mg2+-ATPase,T-AOC and SOD of model group were decreased signifi-cantly,while spleen index and liver index were increased significantly (P<0.01). Compared with model group,WBC,RBC, HGB,HCT,thymus index,the activities of Na+-K+-ATPase,Ca2+-Mg2+-ATPase,T-AOC and SOD of C. sinensis dried body alco-hol extract groups and positive control group were increased significantly;the spleen index and liver index were decreased signifi-cantly in C. sinensis dried body alcohol extract medium-dose and high-dose groups and positive control group (P<0.05 or P<0.01). CONCLUSIONS:C. sinensis dried body alcohol extract shows a certain effect on blood enriching by improving peripheral blood routine and organic function of blood-deficiency model mice.%目的：研究红毛鸡干燥体醇提物对血虚模型小鼠的补血作用。方法：60只小鼠随机分为正常组、模型组、阳性对照组（复方阿胶浆，0.1 ml/10 g）和红毛鸡干燥体醇提物低、中、高剂量组（2.5、5.0、10.0 g/kg），每组10只。除正常组外，其余各组小鼠于实验第2、5天sc乙酰苯肼溶液，第6～9天ip环磷酰胺溶液以复制血虚小鼠模型。从实验第1天起，正常组、模型组小鼠ig等体积蒸馏水，各给药组小鼠ig相应药物，每天1次，连续9 d。给药结束后，采用全自动血细胞分析仪测定各组小鼠外周血中白细胞计数（WBC）、红细胞计数（RBC）、血红蛋白（HGB）含量、血细胞比容（HCT），计算小鼠胸腺指数、脾脏指数和肝脏指数，采用相应试剂盒测定小鼠红细胞膜Na+-K+-ATP酶、Ca2+-Mg2+-ATP酶活力以及肝脏总抗氧化能力（T-AOC）和超氧化物歧化酶（SOD）活力。结果：与正常组比较，模型组小鼠WBC、RBC、HGB、HCT、胸腺指数，Na+-K+-ATP酶、Ca2+-Mg2+-ATP酶活力，T-AOC和SOD活力均显著降低，脾脏指数和肝脏指数显著升高（P＜0.01）。与模型组比较，红毛鸡干燥体醇提物各剂量组和阳性对照组小鼠WBC、RBC、HGB、HCT、胸腺指数，Na+-K+-ATP酶、Ca2+-Mg2+-ATP酶活力，T-AOC和SOD活力均显著升高；红毛鸡干燥体醇提物中、高剂量组及阳性对照组小鼠脾脏指数、肝脏指数均显著降低（P＜0.05或P＜0.01）。结论：红毛鸡干燥体醇提物能改善血虚模型小鼠外周血象和脏器功能，具有一定的补血作用。