维吾尔药木吒栓中石榴皮和石榴花水提取工艺的优化

摘要

OBJECTIVE:To optimize the water extraction technology of Punica granatum peel and flowers in Muzha supposito-ry. METHODS:Using total tannin,ellagic acid contents as evaluation indexes,extration time,times,the amount of solvents as in-vestigation factors,L9(34)orthogonal test was adopted to optimize the water extraction technology of P. granatum flowers and peel. And verification test was conducted. RESULTS:The optimal extraction technology of total tannin and ellagic acid in P. granatum peel and flowers was as follow as 10-fold water reflux extraction for 3 times with 2 herbs,0.5 h every time. The average content of total tannin and ellagic acid extracted from 3 batches of herbs was 151.69 mg/g (RSD=1.15%,n=3),24.59 mg/g (RSD=1.41%,n=3)in the verification test,respectively. CONCLUSIONS:The optimized extraction technology is stable,feasible,and can be used for extracting the total tannin and ellagic acid in P. granatum peel and flowers.%目的:优化维药木吒栓中石榴花与石榴皮的水提取工艺.方法:以总鞣质和鞣花酸含量为评价指标,以提取时间、提取次数和溶剂用量为考察因素,采用L9(34)正交试验优化石榴花、石榴皮的水提取工艺并进行验证试验.结果:石榴花与石榴皮中总鞣质和鞣花酸的最优提取工艺为将2味药材用10倍量水回流提取3次,每次0.5 h;验证试验中3批药材提取出的总鞣质与鞣花酸平均含量分别为151.69、24.59 mg/g(RSD分别为1.15%、1.41%,n=3).结论:优化的提取工艺稳定、可行,可用于木吒栓中石榴花、石榴皮中总鞣质和鞣花酸的提取.