忧遁草枝叶的化学成分及体外抗肿瘤活性研究

摘要

目的:研究忧遁草枝叶的化学成分,并初步考察其体外抗肿瘤活性.方法:采用多种柱色谱技术对忧遁草95%乙醇提取物的乙酸乙酯、正丁醇部位进行分离、纯化,根据理化性质和波谱数据分析、鉴定化合物结构.采用MTT法测定鉴定出的化合物对人肝癌BEL-7402细胞、人胃癌SGC-7901细胞和人慢性髓原白血病K562细胞的体外抑制活性.结果:从忧遁草枝叶中分离鉴定出8个化合物,分别为羽扇豆醇(1)、对香豆酸甲酯(2)、1-O-acyl-3-O-(β-D-galactopyranosyl)-sn-glycerol(3)、(6R,10R)-6,10,14-trimethyl-2-pentadecanone(4)、邻苯二甲酸二(2-乙基-己基)酯(5)、α-D-甲基半乳糖苷(6)、β-D-甲基葡萄糖苷(7)、9-hy-droxy-megastigma-4,7-dien-3-one-9-O-β-D-glucopyranoside(8);化合物1对人肝癌BEL-7402细胞具有弱的生长抑制作用(半数抑制浓度为42.5μmol/L),尚未发现其他7种化合物对上述3种肿瘤细胞有抑制作用.结论:化合物2~8为首次从忧遁草枝叶中分离得到,羽扇豆醇具有弱的抗人肝癌BEL-7402细胞的活性,该研究为忧遁草的质量评价奠定了一定的基础.%OBJECTIVE:To study the chemical constituents of Clinacanthus nutans branches and leaves and its anti-tumor ac-tivity in vitro. METHODS:Varied chromatographic techniques were conducted to separate and purify the ethyl acetate and butanol parts of 95%ethanol extracts of C. nutans,the chemical structures were analyzed and identified according to physicochemical prop-erty and spectral data. MTT method was used to determine the inhibitory activity of identified compounds on human liver cancer BEL-7402 cell lines,gastric cancer SGC-7901 cell lines and chronic myelogenous leukemia K562 cell lines in vitro. RESULTS:Eight compounds isolated from C. nutans branches and leaves were identified as lupeol(1),methyl p-coumarate(2),1-O-acyl-3-O-(β-D-galactopyranosyl)-sn-glycerol(3),(6R,10R)-6,10,14-trimethyl-2-pentadecanone(4),1,2-benzenedicarboxylic acid bis (2-ethylhexyl) ester (5),methyl α-D-galactopyranoside (6),methyl β-D-glucopyranoside (7),9-hydroxy-megastigma-4,7-di-en-3-one-9-O-β-D-glucopyranoside(8). Compound 1 had weak growth inhibition effect on human liver cancer BEL-7402 cell(half inhibitory concentration was 42.5μmol/L),the other 7 compounds had not been found to inhibit the above 3 tumor cell lines. CON-CLUSIONS:Compounds 2-8 are all isolated from C. nutans branches and leaves for the first time,while lupeol has weak growth inhibition effect on human liver cancer BEL-7402 cell. The study has laid the foundation for the quality evaluation of C. nutans.