大理白族自治州不同居群坚龙胆叶中裂环烯醚萜类物质含量的研究

摘要

目的:探讨大理白族自治州(简称大理州)不同居群坚龙胆叶中裂环烯醚萜类物质(龙胆苦苷、獐牙菜苦苷、獐牙菜苷)的含量,为坚龙胆优良品种筛选及其开发应用提供参考.方法:采用高效液相色谱法(HPLC)测定大理州不同居群坚龙胆叶中裂环烯醚萜类物质含量,建立其HPLC指纹图谱,并运用单因素方差分析、总含量聚类分析和主成分分析法对不同居群坚龙胆叶中裂环烯醚萜类物质的含量进行研究.结果:大理州不同居群坚龙胆叶中龙胆苦苷、獐牙菜苦苷、獐牙菜苷的含量及其总含量差异均有统计学意义(P<0.05或P<0. 01);确定了HPLC指纹图谱中12个共有峰,除感通居群外,其余共有峰HPLC指纹图谱相似度均在0. 972以上,并指认了其中龙胆苦苷、獐牙菜苦苷、獐牙菜苷3个与活性相关的特征峰;不同居群坚龙胆叶中龙胆苦苷、獐牙菜苦苷、獐牙菜苷总含量聚类分析和HPLC指纹图谱主成分分析结果相同.结论:大理州不同居群坚龙胆叶中龙胆苦苷、獐牙菜苦苷、獐牙菜苷含量差异较大;除感通居群外,不同居群坚龙胆叶中裂环烯醚萜类物质相似度较高.%OBJECTIVE: To explore the contents of secoiridoid substances (gentiopicroside, swertiamarin, sweroside) in the leaves of Gentiana rigescens from different populations in Dali Bai Autonomous Prefecture (Shorted for Dali prefecture), and to provide reference for the selection of fine varieties, the development and utilization of G.rigescens. METHODS: The contents of secoiridoid substances in the leaves of G. rigescens from different populations in Dali prefecture were determined by HPLC, and the HPLC fingerprint was established. The contents of secoiridoid substances in the leaves of G. rigescens from different populations was studied by using single factor variance analysis, clustering analysis and principal component analysis. RESULTS: There were statistical significance in the contents of gentiamarin, swertiamarin and sweroside and the total contents in the leaves of G. rigescens from different populations in Dali prefecture (P<0. 05 or P<0. 01). Twelve common peaks were found in HPLC fingerprint, and HPLC fingerprint similarities of common peaks except Gantong population were all over 0. 972. Three peaks of them were identified as the characteristic peaks of gentiamarin, swertiamarin and sweroside which were correlated to the activities. The total contents of gentiamarin, swertiamarin and sweroside from different populations obtained by clustering analysis were consistent with that by HPLC fingerprint principal component analysis. CONCLUSIONS: There are great differences in the content of gentiamarin, swertiamarin and sweroside in the leaves of G. rigescens from different populations in Dali prefecture. The similarity of secoiridoid substances in the leaves of G. rigescens from different populations except Gantong population is higher.