HPLC法测定归芍通络合剂中补骨脂素、异补骨脂素的含量

摘要

目的:建立归芍通络合剂中补骨脂素、异补骨脂素的HPLC含量测定方法.方法:色谱柱Inertsil ODC-SP C18(250 mm×4.6 mm,5 μm);流动相:甲醇-水(50:50);流速:0.8 ml·min-1;柱温为40℃;检测波长为246 nm.结果::补骨脂素和异补骨脂素分别在0.041 6～0.249 6 μg(r=0.999 8)和0.040 8～0.244 8 μg(r=0.999 8)范围内具良好的线性关系,回收率分别为98.2%(RSD=1.25%)和99.4%(RSD=1.80%)(n=5).结论:本方法简便,准确,重复性好,可作为归芍通络合剂中补骨脂素、异补骨脂素含量的测定.%Objective: To establish a method for the content determination of psoralen and isopsoralen in Guishao Tongluo mixtures by HPLC. Method: The Inertsil ODC-SP C18 column( 250 mm ×4. 6 mm,5 μm ) was used, the mobile phase was methanol-water( 50 :50 ), the flow rate was 0. 8 ml ? Min~ , the column temperature was 401 and the detection wavelength was 246 nm. Result: The linear range of psoralen and isopsoralen was 0. 041 6 ~0. 249 6 μg( r =0. 999 8 )and 0. 040 8 ~0. 244 8 |xg( r =0. 999 8 )with the average recovery of 98. 2% ,( RSD = 1. 25% )and 99.4% ( RSD = 1. 80% )(n = 5), respectively. Conclusion: The method is simple, accurate and repeatable, and can be used for the content determination of psoralen and isopsoralen in Guishao Tongluo mixtures.