Objective: To establish a specific method for the determination of seven chemicals in antihypertensive traditional Chinese medicine preparations and health products. Method: Nifedipine, atenolol, prazosin hydrochloride, reserpine, clonidine hydro-chloride, captopril and hydrochlorothiazide were determined and analyzed by UPLC-MS/MS. An ACQUITY BEH C18 analysis column (2.1 mm×50 mm ,1.7 μm ) was used with the mobile phase of 0. 1 % formic acid and methanol with gradient elution. The flow rate was 0. 3 ml·min-1 . EIS ion source was used with positive and negative ion monitoring. Result: The standard curves of the seven chemicals showed good linearity over the concentration range of 62.5-2 000.0,65.6-2 100.0, 16.6-530.0,34.5-2 210.0,67.1-2 146.0,33.7-2 159.0, 1 637.3-104 790.0 ng·ml-1 , respectively. The average recoveries of the seven chemicals were within 85. 1% and 106.7% . Conclusion: The method is specific, sensitive,simple and quick,and can be used to detect the seven chemicals in antihypertensive traditional Chinese medicine preparations and health products.%目的:建立降压类中药制剂及保健品中非法添加7种化学药品的检测方法.方法:采用超高效液相色谱-串联四级杆质谱,多反应监测(MRM)模式进行定性和定量检测.采用ACQUITY BEH C18色谱柱(2.1 mm×50 mm,1.7 μm),流动相为0.1%甲酸-甲醇梯度洗脱,流速为0.3 ml·min-1;离子源为ESI源,正负离子检测,对中成药及保健品中添加硝苯地平、阿替洛尔、盐酸哌唑嗪、利血平、盐酸可乐定、卡托普利、氢氯噻嗪进行定性、定量检测.结果:7种化学药品测定的线性范围分别为62.5～2 000.0,65.6～2 100.0,16.6～530.0,34.5～2 210.0,67.1～2 146.0,33.7～2 159.0,1 637.3～104 790.0 ng·ml-1,7种化学药品平均回收率在85.1%～106.7%之间.结论:本方法选择性强、灵敏度高、处理方法简单,测定快速,可用于降压类中成药及保健品中添加7种化学药品的测定.
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