离子色谱法测定浓维磷糖浆中甘油磷酸根及磷酸根的含量

摘要

Objective:To establish ion chromatography(IC) method for determination of the content of sodium glycerophosphate and phosphate salts in concentrated divitamins and sodium phosphate syrup. Methods:An AG19 -HC (50 mm× 4 mm) guard col-umn and an IonPac AS18 -HC (250 mm × 4 mm) ion analysis column were used, potassium hydroxide solution was applied as the mobile phase with gradient elution,and a conductance detector was used with suppression conductometric detection. Results:Sodium glycerophosphate and phosphate could be separated well under the chromatographic conditions. The linear range was 1.054-84.35 μg ·ml-1(r=0.999 4)for sodium glycerophosphate and 0.316 8-25.35 μg·ml-1(r=0.999 8)for phosphate. The average recovery was 99.35% (RSD=1.16%,n=9) and 98.96%(RSD=2.61%,n=9), respectively.Conclusion: The method is simple, rapid and accurate,and can be used for the quality control of concentrated divitamins and sodium phosphate syrup.%目的:建立抑制电导离子色谱法同时测定浓维磷糖浆中有效成分甘油磷酸根及磷酸根的含量.方法:采用AG19 -HC(50 mm×4 mm)保护住和IonPac AS18-HC(250 mm×4 mm)阴离子分析柱,以氢氧化钾溶液梯度淋洗,检测器为电导检测器,检测方式为抑制电导检测.结果:在该色谱条件下,甘油磷酸根峰与磷酸根峰均能有效分离,线性范围分别为1.054 ~84.35 μg·ml-1(r=0.9994)及0.3168~25.35 μg·ml-1(r=0.9998);平均回收率分别为99.35%(RSD=1.16%,n=9)和98.96%(RSD=2.61%,n=9).结论:本法简便,准确,专属性强,有助于更好地控制本品的质量.