高效液相色谱法测定小儿清解冲剂中连翘苷含量

摘要

目的 建立测定小儿清解冲剂中连翘苷含量的高效液相色谱法.方法 采用依利特Hypersil ODS2色谱柱(200film×4.6 mm,5 μm),以乙腈-水(25:75)为流动相,检测波长278 nm,流速为1.0 mL/min.结果 连翘苷进样量与峰面积的线性范围为0.103 9～0.519 5 μg(r=0.999 7),平均回收率为98.95%.RSD为0.68%(n=6).结论 高效液相色谱法简便、准确、重复性好,可用于小儿清解冲剂的质量控制.%Objective To establish a determination method of forsythin in Xiaoerqingjie Granules by HPLC. Methods The determination was carried out on the Hypersil ODS2 column(200 mm ×4.6 mm, 5 μm) with the mobile phase of methanol-H2O(25:75). The detective wavelength was set at 278 nm and the flow rate was 1.0 mL/min. Results The linear range of forsythin was 0. 103 9-0. 519 5 μg( r =0. 999 7).The average recovery rate of forsythin was 98.95％, RSD was 0.68％ (n=6).Conclusion This method is simple, accurate and reproducible, which is suitable for the quality control of Xiaoerqingjie Granules.