高效液相色谱法测定止咳宁嗽胶囊中苦杏仁苷含量

摘要

Objective To establish a HPLC method for the determination of amygdalin in Zhikeningsou Capsule.Methods The Zorbax Eclipse XDB-C18 column(150mm×4.6mm,5μm) was used with methanol-water(20:80)as the mobile phase.The UV detection wavelength was at 218 mm and the flow rate was 1.0mL/min.Results The linear range of amygdalin was 7.16-71.6μg/mL(r＝0.9995).The average recovery rate was 99.21％ with RSD of 0.48％(n＝6).Conclusion The method is highly specific,simple to operate,and can be used to control the quality of this product.%目的 建立测定止咳宁嗽胶囊中苦杏仁苷含量的高效液相色谱法.方法 用Zorbax Eclipse XDB-C18色谱柱(150 mm×4.6 mm,5μm),流动相为甲醇-水(20:80),检测波长为218 nm,流速为1.0 mL/min.结果 苦杏仁苷的质量浓度线性范围为7.16～71.6 μg/mL(r=0.999 5),平均回收率为99.21%,RSD为0.48%(n=6).结论 该方法专属性强、操作简便,可用于产品的质量控制.