甘草中异甘草素和甘草素含量测定方法研究

摘要

Objective To establish the method for simultaneously determining the isoliquiritigenin and liquiritigenin contents in gly-cyrrhiza uralensis Fisch. Methods The isocratic elution and the detection wavelength time sequence sampling by HPLC were adopt-ed. The mobile phase was methanol - water(45 : 55),the detection wavelength was 276 nm for 0 - 25 min and 360 mn for 25 - 70 min and the flow rate was 0. 8 mL. min - 1. Under these conditions,the isoliquiritigenin and liquiritigenin contents in glycyrrhiza uralensis Fisch were simultaneously determined. Results Isoliquiritigenin(0. 028 - 0. 28 μg)and liquiritigenin(0. 064 - 0. 64 μg)had good lin-ear relation. The average recovery rates of the two components were 97. 92% - 98. 27% with RSD of 1. 29% - 1. 67% . Conclusion The method is accurate and highly sensitive with good reproducibility and easy operation,and can provide the reference data for the e-valuation of glycyrrhiza uralensis Fisch medicinal material.%目的：建立同时测定甘草中异甘草素和甘草素含量的方法。方法采用高效液相色谱（HPLC）等度洗脱和检测波长时间序列采样的方法，流动相为甲醇-水=(45：55)，检测波长0~25 min 时为276 nm，25~70 min 时为360 mn，流速为0.8 mL / min，同时测定甘草中异甘草素和甘草素的含量。结果异甘草素和甘草素进样量线性范围分别为0.028~0.28μg 和0.064~0.64μg，平均加样回收率分别为97.92%和98.27%，RSD 分别为1.29%和1.67%。结论该方法准确、灵敏度高、重现性好、操作简单，可为甘草药材的质量评价提供参考。