人参皂苷Rb1对人白血病KG1a细胞增殖及凋亡抑制作用研究

摘要

目的 探讨人参皂苷Rb1对人白血病细胞KG1a增殖及凋亡抑制作用的机制.方法 取对数生长期的KG1a细胞,空白对照组常规培养;人参皂苷单体 Rb1 组分别加入20,40,80,160 μmol/L的Rb1.采用CCK-8法检测KG1a细胞增殖抑制情况;流式细胞术测定细胞凋亡情况;Western Blot法检测去乙酰化酶3(HDAC3)及抗凋亡蛋白 Bcl-2,Caspase-3的表达情况.结果 人参皂苷 Rb1在体外对KG1a细胞有明显的增殖抑制作用,在20~160 μmol/L浓度范围内抑制作用呈浓度依赖性,且在48 h时抑制最明显;流式细胞术显示,随着处理药物浓度的增加,ROS水平显著增加( P<0. 05),KG1a细胞凋亡率显著升高,其中早期凋亡细胞及晚期凋亡细胞百分比与0 μmol/L组相比,均有显著性差异( P<0. 05);Western Blot结果显示,随着药物浓度增加,HDAC3的表达显著降低( P<0. 05),而 Bcl-2,Caspase-3 表达增加.结论 人参皂苷 Rb1 可抑制 KGla 细胞增殖,其机制可能是通过下调 HDAC3 促进KG1a细胞凋亡而实现.%Objective To investigate the mechanism of ginsenoside Rb1 on the proliferation and apoptosis of human leukemia KG1a cell. Methods KG1a cells in the logarithmic growth phase were taken as the blank control group. The Rb1 group of the ginsenoside monomer was added to 20,40,80,160 Rb1 μmol/L. CCK-8 method was used to detect the proliferation of KG1a cells;apoptosis was detected by flow cytometry;Western Blot was used to detect the express of HDAC3,Bcl-2 and Caspase-3. Results The inhibition ef-fect of Rb1 on the proliferation of KG1a cells was significantly inhibited in vitro;in the range of 20-160 μmol/L Rb1,the inhibition effect was concentration dependent,and the inhibition was most obvious at 48 h;flow cytometry showed that with the increase of drug concentration,ROS level increased significantly ( P < 0. 05),and the apoptosis rate of KG1a cells was significantly increased,and the percentage of apoptotic cells and apoptotic cells was significantly different from that of the 0 μg/mL group( P < 0. 05);Western Blot showed that the expression of HDAC3 decreased significantly with the increase of drug concentration( P < 0. 05),while the expression of Caspase-3 and Bcl-2 increased. Conclusion Ginsenoside Rb1 can inhibit the proliferation of KG1a cells,and the inhibition may be achieved by promoting the apoptosis of KG1a cells.