内质网应激PERK－ATF4－CHOP通路在苦参碱诱导视网膜母细胞瘤细胞凋亡中的作用

摘要

Objective To analyze the role of endoplasmic reticulum stress PERK-ATF4-CHOP pathway in matrine induced retinoblastoma cells apoptosis. Methods Different concentrations of 0, 1. 0, 2. 0, 4. 0 g/L matrine was used to treat the HOX-Rb44 cells for 24 h,the cell viability was determined by MTT assay, HOX-RB44 apoptosis situation was determined by ow cytometry,western blot analysis was used to dectect the Bax,Bcl-2,GRP78,GRP94,AFT4,peIF2α and CHOP expression. Results As the increase of concentration of ma-trine,HOX-Rb44 apoptosis rate increased significantly ( P < 0. 05 ) , GRP78 and GRP94 increased significantly, and ATF and PERK downstream peIF2α and CHOP were also increased significantly. Conclusion Matrine could promoted HOX-Rb44 cell apoptosis, endo-plasmic reticulum stress, where PERK-ATF4-CHOP pathway may play an important role.%目的：探讨内质网应激 PERK－ATF4－CHOP通路在苦参碱诱导视网膜母细胞瘤细胞凋亡中的作用。方法采用0，1.0，2.0，4.0 g/L苦参碱处理HOX－Rb44细胞24 h，MTT、流式细胞术分析细胞增殖活性及凋亡情况，Western Blot检测凋亡相关蛋白Bax，Bcl－2，内质网应激相关蛋白GRP94，GRP78，ATF4以及PERK下游peIF2α及CHOP蛋白表达水平。结果随着苦参碱质量浓度的增加，HOX－Rb44细胞增殖抑制增加，凋亡率显著升高（ P＜0.05），GRP78与GRP94的表达水平逐渐升高，ATF及PERK下游peIF2α及CHOP蛋白表达升高。结论苦参碱处理视网膜母细胞瘤细胞能诱导细胞凋亡，内质网应激、PERK－ATF4－CHOP通路可能在其中有重要作用。