大鼠激素性股骨头坏死的2种造模方法比较

摘要

Objective To compare the reliability and success rate of the different intraperitoneal injection of dexamethasone regimen in glucocorticoid-induced avascular necrosis in rats model,and provide the basic data for setting up rat models of steroid-induced avas-cular necrosis of femoral head.Methods 30 male Wistar rats were randomly divided into group A (10)and group B (10),group C (10).Group A was given dexamethasone sodium phosphate injection 20 mg/(kg·d),1 time/week for 8 weeks;group B was given dexamethasone sodium phosphate injection 10 mL/(kg·d),2 times/week for 8 weeks;group C was given 0.9% chloride 20 mL/(kg·d),1 time/week for 8 weeks.Results No abnormalities were found in double hip X-ray examination of the three groups,but significant differences can be found on pathological section,the differences confirmed that building hormone osteonecrosis is successful and the degree of femoral head necrosis of Group B is more serious than group A.Conclusion Both methods(intraperitoneal injection of dexamethasone,20 mg/(kg·d),1 time/week,for 8 weeks and intraperitoneal injection of dexamethasone,10 mL/(kg·d),2 times/week,for 8 weeks) can successfully established early stage rat models of glucocorticoid-induced avascular,and the latter method is better than the previous one.%目的：比较不同地塞米松用药方案腹腔注射建立大鼠激素性股骨头坏死模型的可靠性及成功率，为大鼠激素性股骨头坏死模型的建立方法提供基本数据。方法将30只雄性Wistar大鼠随机分为A组、B组、C组，各10只。A组给予地塞米松磷酸钠注射液20 mg／(kg·d)腹腔注射，1次／周；B组给予地塞米松磷酸钠注射液10 mL／(kg·d)腹腔注射，2次／周；C组给予0．9％氯化钠注射液20 mL／(kg·d )腹腔注射，1次／周；均持续8周。于首次给药8周末摄双髋正位X线检查，次日处死取双侧股骨头标本，行组织形态学检查。结果 A、B、C3组大鼠股骨头双髋正位X线检查均未发现异常，但在病理切片上可发现显著性差异，证实激素性股骨头坏死造模成功，而B组股骨头坏死改变较A组明显严重。结论腹腔注射地塞米松20 mg／(kg·d)，1次／周，持续8周的方案和地塞米松10 mL／(kg·d )，2次／周，持续8周的方案均能成功建立大鼠激素性股骨头坏死的早期模型，但后一种造模方案更优。