高效液相色谱法测定黄芩苷脂质体药物包封率

摘要

目的：建立测定黄芩苷脂质体中药物包封率的高效液相色谱（HPLC）法。方法色谱柱为Fortis Xi C18柱（250 mm ×4.6 mm，5μm )，流动相为乙腈-0.2%磷酸溶液（35∶65），柱温为25℃，流速为1.0 mL/min，检测波长为278 nm。结果黄芩苷质量浓度在6~100μg/mL范围内与峰面积线性关系良好（ r=0.9998，n=5），平均回收率为99.51%，RSD为2.09%（ n=9）。结论该法准确、简便、快速，可用于黄芩苷脂质体包封率的测定。%Objective To establish an HPLC method for the determination of drug encapsulation efficiency of baicalin liposomes. Methods The chromatographic column was Fortis Xi C18 column ( 250 mm × 4. 6 mm,5 μm ) ,the mobile phase was acetonitrile-0. 2%phosphoric acid solution(35 ∶65),the column temperature was 25 ℃,the flow rate was 1. 0 mL/min,the UV detection wavelength was 278 nm. Results The baicalin concentration showed a good linear relationship in the range of 6-100 μg/mL( r=0. 999 8,n=5). The average recovery rate was 99. 51%,RSD was 2. 09%( n=9 ) . Conclusion This method is accurate,simple,rapid and can be used for the determination of baicalin liposomes encapsulation efficiency.