目的：建立测定呋喃西林含量的高效液相色谱（HPLC）法，并与紫外－可见分光光度（UV）法进行比较。方法 HPLC法以十八烷基硅烷键合硅胶为填充剂，以0.125%三乙胺缓冲液－乙腈（85：15）为流动相，流速为1 mL/min，检测波长为365 nm；UV法检测波长为375 nm。结果 HPLC法测得3份样品含量分别为标示含量的90.21%，90.73%，89.75%；UV法测得3份样品含量分别为标示含量的95.72%，94.09%，94.73%。结论 UV法比HPLC法测定样品的含量稍高，但HPLC法更适合作为呋喃西林溶液含量测定的方法。%Objective To establish a high performance liquid chromatography ( HPLC ) method for determining the nitrofurazone content and to compare it with the ultraviolet ( UV ) method. Methods With octadecylsilane chemically bonded silica as the filler,0. 125% tri-ethylamine buffer solution-acetonitrile solution (85 :15) was used as the mobile phase. The flow rate was 1 mL/min and the wave-length of detection was 375nm by the UV method. Results The nitrofurazone contents of three nitrofurazone solution samples deter-mined by the HPLC method were 90. 21%,90. 73% and 89. 75% of the labeled amount,while those determined by the UV method were 95. 72%,94. 09% and 94. 73% of the labeled amount respectively. Conclusion The determined content of nitrofurazone solution samples by the UV method is slightly higher than that determined by the HPLC method,but the HPLC method is more suitable as the content determination method for the nitrofurazone solution.
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