高效液相色谱法测定槐枝合剂的防腐剂含量

摘要

Objective To establish an HPLC method for effective control of the content of sodium benzoate in Huaizhi Mixture of hospital preparation. Methods The Agilent XDB-C18 column(150 mm × 4. 6 mm, 5 μm) was adopted, the mobile phase was 0. 05 mol/L sodium dihydrogen phosphate solution ( pH=3. 0 with phosphoric acid ) -acetonitrile ( 65 :35 ) , the flow rate was 1 mL/min, the detection wave length was 248 nm and the column temperature was 25 ℃. Results The peak of sodium benzoate and other peaks in samples could be separated from baseline. The regression equation was Y=3531. 4 X+2. 683 ( r=0. 9999, n=6 ) , the sodium benzoate showed a good linear in the concentration range of 0. 02-0. 16 g/L, the average recovery was 98. 43%, and RSD was 1. 42%( n=6 ) . Conclusion The method can be used for the quality control of sodium benzoate in Huaizhi Mixture.%目的 建立有效控制医院制剂槐枝合剂中苯甲酸钠含量的高效液相色谱(HPLC)法.方法 色谱柱采用Agilent XDB-C18柱(150 mm×4.6 mm,5μm),流动相为0.05 mol/L磷酸二氢钠溶液(用磷酸调pH为3.0)-乙腈(65:35),流速1 mL/min,检测波长248 nm,柱温25℃.结果 苯甲酸钠峰与其他峰能达到基线分离,并能排除辅料的干扰,线性回归方程为Y=3531.4 X+2.683(r=0.9999,n=6),苯甲酸钠质量浓度在0.02~0.16 g/L范围内与峰面积积分值线性关系良好,平均回收率为98.43%,RSD为1.42%(n=6).结论 该方法可用于槐枝合剂的质量控制.