冷冻溶剂结晶法分离纯化混合脂肪酸中的亚油酸

摘要

Linoleic acid was separated from mixed fatty acids by frozen solvent crystallization, and the relative contents of fatty acids were determined by GC - MS. The optimal conditions were as follows;acetone used as a solvent, volume ratio of fatty acids to solvent 1:1, crystallization temperature gradient 0 , - 25 , -35 t, freezing time 6 h at each temperature. The content of linoleic acid increased from 50.58% to 85.33% ,and the yield was 68%.%以冷冻溶剂结晶法分离纯化混合脂肪酸中的亚油酸,用气-质联用测定产物中脂肪酸相对含量.研究确定最佳工艺务件为:以丙酮为溶剂,V(混合脂肪酸)∶V(溶剂)=1∶1,结晶温度梯度为O、-25、-35℃,每个温度下冷冻6h.最佳条件下,可将亚油酸的含量从50.58％提高到85.33％,得率为68％.