研究光照强度和硝酸钾质量浓度对微绿球藻生物量和油脂含量的影响,通过两步培养法培养微绿球藻提高其生物量和油脂含量.结果表明:在硝酸钾质量浓度100 mg/L条件下,当光照强度为1 940～2 060 lx时,培养12d,微绿球藻的生物量和油脂含量最高,分别为0.68 g/L和16.28％;低光照强度(1 940 ～2 060 lx)下,在不添加硝酸钾时,微绿球藻的生物量最低,为0.39 g/L,油脂含量最高,为22.62％;随着硝酸钾质量浓度的增加,微绿球藻的生物量逐渐增加,而油脂含量逐渐下降;两步培养法可以促进微绿球藻的油脂合成,油脂含量和油脂产量分别达到30.50％和0.15 g/L.%The effects of light intensity and mass concentration of potassium nitrate on the biomass and oil content of Nannochloris oculata were investigated,and the microalgae was cultivated by two-stage cultivation method to enhance the biomass and oil content.The results showed that Nannochloris oculata achieved the highest biomass of 0.68 g/L and oil content of 16.28％ in the light intensity of 1 940-2 060 lx and mass concentration of potassium nitrate 100 mg/L for 12 d of cultivation.The lowest biomass and highest oil content of Nannochloris oculata in the nitrogen-deficient medium were 0.39 g/L and 22.62％,respectively at low light intensity (1 940-2 060 lx).An increase in the mass concentration of potassium nitrate resulted in an increase in the biomass and a decrease in the oil content of Nannochloris oculata.Two-step cultivation method promoted the oil synthesis of Nannochloris oculata,and the oil content and oil yield were 30.50％ and 0.15 g/L,respectively.
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