同位素稀释-GC-MS/MS测定植物油中邻苯二甲酸酯

摘要

Sixteen phthalate esters (PAEs) were simultaneously determined in vegetable oil using gas chromatography-tandem mass spectrometry (GC-MS/MS) with isotope dilution.The samples were extracted with acetonitrile saturated by n-hexane and cleaned up with solid phase extraction column,then determined by GC-MS/MS using internal standard method for quantitative analysis.The results showed that the method had a good linear relationship with correlation coefficient above 0.99,and the detection limit and quantitation limit were 3.0 μg/kg and 10.0 μg/kg,respectively;adding PAEs mixed standard solution with three levels 10,50,100 μg/kg to vegetable oil,the mean recoveries of sixteen PAEs in vegetable oil were 82.3％-104.5％,and the relative standard deviations were 1.3％-10.8％.The method had high sensitivity,little interference and good precision,which was suitable for the determination of PAEs in vegetable oil.%采用同位素稀释-气相色谱-质谱/质谱(GC-MS/MS)技术同时测定植物油中16种邻苯二甲酸酯类塑化剂.样品用正己烷饱和的乙腈提取,经固相萃取小柱净化,采用GC-MS/MS测定,内标法定量.结果表明:该方法线性关系良好,相关系数在0.99以上,检出限和定量限分别为3.0 μg/kg和10.0 μg/kg;添加10、50、100 μg/kg 3个水平的混合标准溶液,植物油中16种邻苯二甲酸酯的平均回收率在82.3％～104.5％,相对标准偏差在1.3％～10.8％.该方法灵敏度高、干扰小、定量结果准确可靠,适合植物油中邻苯二甲酸酯类塑化剂的检测.