间质性肺疾病患者呼出气冷凝液中8-异前列腺素及白三烯B4的水平及临床意义

摘要

Objective To investigate the levels of 8-isoprostane(8-iso-PG) and leukotriene B4(LTB4) in exhaled breath condensate(EBC)and its clinical significance in interstitial lung disease(ILD). Methods 20 ILD patients in our depart-ment from August 2012 to December 2013 were selected as the ILD group,20 healthy subjects matched with age and body mass index were selected as control group.The levels of 8-iso-PG and LTB4 in EBC were measured by ELISA. Results The level of 8-iso-PG in ILD group was (9.69+2.8) ng/L,which was higher than that in control group(4.86+1.9) ng/L, with significant difference (P﹤0.05).The level of LTB4 in EBC of ILD group was (15.46+6.6) ng/L,higher than that in control group (8.55+4.4) ng/L,the difference was statistically significant(P﹤0.01).The specificity of LTB4 and 8-iso-PG was 85%respectively,and the sensitivity was 90% and 60% respectively. Conclusion 8-iso-PG and LTB4 increases in EBC of ILD patients.Measuring biomarkers in EBC may be useful to evaluate inflammatory response of ILD.%目的：探讨间质性肺疾病（ILD）患者呼出气冷凝液（EBC）中8-异前列腺素（8-iso-PG）及白三烯B4（LTB4）的水平及其临床意义。方法选取2012年8月~2013年12月在我科住院的20例ILD患者为ILD组,20例年龄、性别、体重指数等均相匹配的健康体检者为对照组,采用ELISA检测EBC中8-iso-PG及LTB4水平。结果 ILD组EBC中8-iso-PG水平为（9.69±2.8）ng／L,高于对照组的（4.86±1.9）ng／L,差异有统计学意义（P﹤0.05）；ILD组EBC中LTB4水平为（15.46±6.6）ng／L,高于对照组的（8.55±4.4）ng／L,差异有统计学意义（P﹤0.01）。8-iso-PG、LTB4的特异度均为85％,敏感度分别为90％、60％。结论8-iso-PG及LTB4在ILD患者EBC中升高,测定EBC生物标志物或可用于评价ILD炎症反应。