绝经前后三阴性乳腺癌患者差异蛋白分析

摘要

目的：应用iTRAQ技术分析绝经前后三阴性乳腺癌组织差异蛋白的表达情况，旨在探讨绝经对三阴性乳腺癌发生的影响。方法选择绝经前及绝经后各8例经病理确诊的三阴性乳腺癌患者，应用iTRAQ技术分析差异蛋白显著性功能、差异蛋白pathway并进行差异蛋白验证。结果(1﹚绝经前差异蛋白相互作用组较集中，而绝经后的差异蛋白较多，且分布较散。(2﹚绝经前癌组织的差异蛋白存在于5种差异显著途径中；绝经后癌组织的差异蛋白存在于15种差异显著途径中。(3﹚与癌旁组织相比，绝经前共有214种显著差异蛋白，绝经后共有360种显著差异蛋白。绝经前的三阴性乳腺癌组织中上调蛋白81种，下调蛋白133种，绝经后上调蛋白157种类，下调203种。结论利用iTRAQ技术发现，绝经前后三阴性乳腺癌患者差异蛋白表达具有一定的特殊性，说明不同雌激素环境下三阴性乳腺癌可能存在不同发病机制，可能成为治疗TNBC的新靶点。%Objective To analyze the differential protein expression of three negative breast cancer tissue before and after menopause application of iTRAQ technology, to explore the effects of menopause on three negative breast cancer. Methods Selected premenopausal and postmenopausal women with 8 cases in each by negative pathological diagnosis, differential protein analysis on significant function, difference of protein pathway and difference of protein verify were analyzed by the application iTRAQ technology. Results (1﹚Differential protein interactome was relatively centralized premenopausal, and postmenopausal differences of protein was much, and the distribution of the dispersed. (2﹚In pre menopausal cancer tissue protein differences existed in 5 of the significant difference in differences approach; post-menopausal cancer tissue protein existsed in 15 significantly different pathways. (3﹚Tumor adjacent tissues compared with premenopausal had a total of 214 significant difference proteins, postmenopausal had a total of 360 significant differ-ence proteins. The upregul ated proteins in 81 kinds of premenopausal three negative breast cancer tissues, 133 down regμl ated proteins, postmenopausal up-regμl ated protein 157 types, down 203. Conclusion Using iTRAQ technology to found that the expression has a certain particμl arity differences in three negative breast cancer before and after menopause, indicate three negative breast cancer may have different pathogenesis in the different estrogen environment, may be a new target for treatment of TNBC.