目的 探讨慢性乙型肝炎患者外周血CD4+CD25+调节性T细胞(CD4+ CD25+ Treg)检测的临床意义。方法 慢性HBV感染者48例（慢性HBV携带者10例为慢性HBV携带者组、慢性乙型肝炎18例慢性乙型肝炎组和乙肝肝硬化为乙肝肝硬化组20例），以流式细胞仪检测血CD4+CD25+Treg细胞频率，实时荧光定量聚合酶链反应检测血HBV DNA载量。结果 血CD4+CD25+Treg细胞百分率：慢性HBV携带者组为(6.72±2.60)％、慢性乙型肝炎组为(8.56±3.12)％、乙肝肝硬化组为(11.59±4.34)％，组间差异有统计学意义（均P＜0.05）。血HBV DNA载量：慢性HBV携带者组为1.7 ×106拷贝/ml、慢性乙型肝炎组为4.3 ×105拷贝/ml、乙肝肝硬化组为6.8 ×104拷贝/ml，组间差异有统计学意义(P＜0.01)；慢性肝病患者血CD4+CD25+调节性T细胞百分率与HBV DNA滴度正相关。结论 慢性肝病患者血Treg细胞频率与HBVDNA滴度正相关，提示Treg细胞在慢性乙型肝炎致病机制中起重要作用。%Objective To investigate the clinical value of circulating T regulatory cell (Treg) in patients with chronic hepatitis B (CHB). Methods The circulating Treg frequency and the levels of HBV replication in 48 patients with CHB, including 10 HBV carriers, 18 CHB, and 20 liver cirrhosis ( LC), were analyzed by fluorescence-activated cell sorting (FACS) and by real-time Polymerase Chain Reaction ( PCR), respectively. Results Tregs frequency was (11.59 ±4.34)％ (P ＜0.05) in LC and significantly higher than that in CHB (8.56 ±3.12) ％ or in HBV carries (6.72 ± 2.60)％. HBV DNA copies was 1.7 × 106 copies/ml (P ＜ 0.01 ) in HBV carriers and significantly higher than that in CHB (4.3 × 105 ) copies/ml or in LC (6.8 × 104 ) copies/ml. Significant relationship was found between Treg frequency and HBV DNA copies. Conclusions Circulating Treg would play an important role in CHB infection with correlated closely with the viral replication.
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