hOGG1基因多态性与胃癌遗传易感性的关系

摘要

Objective To investigate the relationship between polymorphism in human 8-oxoguanine DNA glycosylase 1(hOGG1) and xeroderma pigmentosum group D (XPD) and genetic susceptibility to gastric cancer. Methods The peripheral blood samples of 98 cases of gastric cancer and 80 non-tumor volunteers(control group) in Zhengzhou and Kaifeng of Henan were collected, the peripheral DNA repair enzyme gene polymorphism in gastric cancer populations were detected by using polymerase chain reaction restriction fragment length polymorphism(PCR-RFLP), and it's relationship with genetic susceptibility to cancer was analyzed. Results There were significant differences in genotypes frequency of hOGG1Ser326Cys and XPD Lys751Gln between gastric cancer group and control group (P < 0.05). Lys751Gln genotype had the 1.7 times inceased risk of developing gastric cancer (OR=1.706, 95%CI=0.341 ~ 2.462,P=0.002). The interactions between hOGG1Ser 326Cys polymorphism and alcohol and between XPDLys751Gln polymorphism and alcohol increased the onset risk of gastric cancer(S>1, API=0.38). Conclusion The Lys751Gln genotype and susceptibility to gastric cancer in individuals can be relevant. The interaction between carrying Cys or Gln gene-gene and alcohol can increase the susceptibility to gastric cancer.%目的：探讨人类8-羟基鸟嘌呤DNA糖苷酶1（hOGG 1）基因多态性与胃癌遗传易感性的关系。方法收集河南郑州和开封地区98例胃癌患者和80例非肿瘤对照组志愿者外周血样，应用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）检测法检测胃癌人群的外周血中DNA损伤修复酶基因多态性，分析其与肿瘤遗传易感性的关系。结果 hOGG1Ser326Cys基因的各基因型频率在胃癌组和对照组间的分布差异有统计学意义（P＜0.05）。携带Cys326Cys基因型者胃癌的发病风险增加1.7倍（OR=1.706，95%CI=0.341～2.462，P=0.002）。hOGG1Ser 326Cys基因多态性与酒的交互作用增加胃癌的发病风险（S＞1，API=0.38）。结论Cys326Cys基因型是胃癌发病的危险基因型，携带Cys易感基因与饮酒交互作用时可能增加患胃癌的易感性。