裂褶菌子实体中多糖及蛋白质含量的测定

摘要

Objective Set up Schizophyllumcommune sporophore polysaccharide and protein content determination method. Methods Use spectrophotometry to determine polysaccharide and protein content, detection wavelength is 479nm and 595nm. Results Polysaccharide concentration possesses good linear relationship between 0.012-0.010mg/mL, regression equation is Y=23.0753X-0.1733, r=0.9998, sporophore polysaccharide content is 7.26%; protein concentration possesses good linear relationship between 0.0078-0.0321mg/mL, regression equation is Y=20.0830X+0.1451, r=0.9997, sporophore total protein is 0.2631%. Conclusion The method set up by the research is simple, fast, accurate and has good repeatability which provides basis for Schizophyllumcommune sporophore polysaccharide and protein content determination.%目的：建立裂褶菌子实体中多糖、蛋白质含量的测定方法。方法采用分光光度法测定多糖和总蛋白含量，检测波长分别为479nm和595nm。结果多糖浓度在0.012~0.040mg/mL范围内具有良好的线性关系，回归方程为Y=23.0753X-0.1733，r=0.9998，子实体多糖含量为7.26%；蛋白浓度在0.0078~0.0321mg/mL范围内具有良好的线性关系，回归方程为Y=20.0830X+0.1451，r=0.9997，子实体总蛋白含量为0.2631%。结论本研究建立的方法简便、快速、准确，重复性好，为裂褶菌子实体多糖和蛋白质含量的测定提供了依据。