膝骨性关节炎患者关节液中MMP-7、MMP-9、TIMP-1、TNF-α的表达及意义

摘要

目的：观察并探讨膝关节骨性关节炎患者膝关节液中MMP-7、MMP-9、TIMP-1、TNF-α水平与病变程度的关系。方法膝关节骨性关节炎患者97例，根据患者的X线片，把OA的严重程度分为0～Ⅳ级，并进行WOMAC骨关节炎指数评分，检测膝关节液中MMP-7、MMP-9、TIMP-1蛋白表达量及TNF-α水平，以健康志愿者10例为正常对照组。结果骨性关节炎患者膝关节液中MMP-7、MMP-9、TIMP-1、TNF-α水平均高于正常对照组（P＜0.05），随着病变程度加重，膝关节液中MMP-7、MMP-9、TIMP-1、TNF-α水平显著增加。结论 MMP-7、MMP-9、TIMP-1、TNF-α水平在骨性关节炎患者膝关节液中均升高，与病变程度密切相关，检测这些指标有助于对膝关节骨性关节炎病情的判断。%Objective To explore the relationship between the levels of synovial fluid matrix metalloproteinase-7 (MMP-7), matrix metalloproteinase-9(MMP-9), matrix metalloproteinase inhibitor-1 (TIMP-1), tumor necrosis factor-α with the severity of knee osteoarthritis (KOA).Met hods According to the X-ray, 97 knee osteoarthritis patients, were divided into 0 to IV stages based on the severity of KOA; and WOMAC osteoarthritis index scores were evaluated. Another 10 healthy volunteers were selected as the control group. MMP-7 , MMP-9, TIMP-1 and TNF-α level in knee joint fluid were all detected. Results MMP-7, MMP-9, TIMP-1 and TNF-α levels of synovial fluid in KOA group were significantly higher than those in the control group(P<0.05).The levels of MMP-7,MMP-9, TIMP-1and TNF-α in the synovial fluid were significantly increased with the aggravation of the lesions.Conclusion MMP-7, MMP-9,TIMP-1 and TNF-α level in the knee joint fluid increased in patients with osteoarthritis and were closely related with the extent of the lesions; so detection of these indicators will be helpful to judge the condition of osteoarthritis of the knee.