三维共培养模型下人脐静脉内皮细胞对鼻咽癌CNE2细胞侵袭、迁移及增殖能力的影响

摘要

目的：探讨三维共培养下人脐静脉内皮细胞（HUVEC）对CNE2细胞侵袭、迁移及增殖能力的影响。方法采用Transwell小室对HUVEC细胞和CNE2细胞进行共培养，CCK-8法比较两组实验中CNE2细胞增殖能力，细胞计数法比较细胞迁移能力，以及Matrigel胶法比较侵袭能力。结果HUVEC与CNE2共培养条件下，共培养组迁移能力显著高于非共培养组（P＜0.01）。共培养组侵袭能力显著高于非共培养组（P＜0.01）。在增殖实验中，共培养组和非共培养组的OD值第3天，第4天及第5天，共培养组的细胞增殖率显著高于非共培养组（P＜0.05）。结论在三维共培养条件下，HUVEC对CNE2鼻咽癌细胞的侵袭、迁移及增殖能力有促进作用。%ObjectiveTo explore the influence of human umbilical vein endothelial cells(HUVEC) under the three-dimensional co culture model to invasion, migration and proliferation of nasopharyngeal carcinoma cell line CNE2.Methods Transwell was used to give co culture of HUVEC cells and CNE2 cells.CCK-8 was used to compare the CNE2 cell proliferation.Cell counting method was used to compare the cell migration ability.Matrigel method was used to compare the invasive ability. ResultsUnder the co culture condition of CNE2 and HUVEC,the migration ability of co culture group was significantly higher than that of non co culture group(P<0.01).The invasive ability of the co culture group was significantly higher than that in the non co culture group(P<0.01).In the proliferation experiment,On the third day,fourth day and fifth day,the cell proliferation rate was significantly higher than that of non co culture co culture group(P<0.05).ConclusionUnder three dimensional co culture conditions,HUVEC can promote the invasion,migration and proliferation of CNE2 nasopharyngeal carcinoma cells.