生脉饮对TNF-α诱导的大鼠心肌成纤维细胞胶原合成的影响

摘要

目的 探讨生脉饮对肿瘤坏死因子(tumor necrosis factor α,TNF-α)诱导的新生大鼠心肌成纤维细胞(cardiac fibroblasts,CFs)胶原合成的影响及其机制.方法 用不同浓度生脉饮载药血清与TNF-α处理体外培养的CFs,采用分光光度法检测细胞培养上清液中羟脯氨酸含量,采用实时定量RT-PCR检测MMP1和TIMP1 mRNA的表达水平.结果 TNF-α处理组羟脯氨酸含量较空白对照组明显升高;与TNF-α组比较,生脉饮口服液血清中羟脯氨酸含量降低.与空白对照组比较,TNF-α组MMP1和TIMP1 mRNA表达上调;生脉饮载药血清组MMP1较TNF-α组显著升高,而TIPM1显著降低,差异有统计学意义(均P < 0.05),并呈浓度依赖性.结论 生脉饮对TNF-α诱导的CFs 胶原合成具有抑制作用,下调MMP1和上调TIPM1 mRNA表达可能是其作用的机制之一.%Objective To explore the effect and mechanism of Shengmai Oral Liquid on collagen synthesis of neonatal rat cardiac fibroblasts (CFs) induced by tumor necrosis factor (tumor necrosis factor α, TNF-α). Methods Cardiac fibroblasts (CFs) were treated with different concentrations of Shengmai Oral Liquid drug-loaded serum plus TNF-α treatment in vitro. Hydroxyproline content, MMP1 mRNA and TIMP1 mRNA expression were assayed by spectrophotometry and real-time quantitative reverse transcription-PCR, respectively. Results The content of hydroxyproline in cardiac fibroblasts treated with TNF-α was higher than the control group. The content of hydroxyproline was reduced in Shengmai Oral Liquid drug-loaded serum group compared with the TNF-α group. MMP1 and TIMP1 mRNA expression was up-regulated expression in TNF-α groups compared with the control group. MMP1 mRNA expression was up-regulated and TIMP1 was down-regulated in Shengmai Oral Liquid drug-loaded serum group compared with the TNF-α group. Conclusion The results reveal that Shengmai Oral Liquid can inhibit the collagen synthesis in cardiac fibroblasts, and may correlate with down-regulate expression of TIMP1 mRNA and up-regulate expression of MMP1 mRNA.