类风湿关节炎及系统性红斑狼疮患者血液免疫指标的分析与比较

摘要

Objective To analyze and compare the serum immune index of rheumatoid arthritis and systemic lupus erythematosus and to achieve an accurate diagnosis of these two kinds of autoimmune diseases. Methods 46 cases of RA patients were selected as RA group and 40 cases of SLE patients were selected as SLE group. 30 cases of healthy persons as the control group, indirect immunofluorescence assay was used to detect the serum ANA, Western blot was used to detect the ENA polypeptide antibody and ds-DNA, the positive rate of variety of antibodies in RA group, SLE group and control group. Results In RA group, the positive rate of ANA was 37.0%, the positive rate of anti ds-DNA antibody was 13.0%, the positive rate of anti-ENA peptide antibody was 19.6%, positive rate by joint detection was 50.0%. In SLE group, the positive rate were 92.5%, 70.0%, 87.5% and 95.0% correspondingly. Conclusion Patients with autoimmune disease always have various auto antibodies, joint detection of ANA, anti ds-DNA and anti-ENA polypeptide antibody can improve the detection rate, they have the advantage of diagnosis of autoimmune diseases, also can avoid misdiagnosis and missed diagnosis.%目的 分析和比较类风湿关节炎(rheumatoid arthritis,RA)及系统性红斑狼疮(systemic lupus erythematosus,SLE)患者血液中的免疫指标,从而实现对这两种自身免疫性疾病的准确诊断.方法 分别选择RA患者46例为RA组、SLE患者40例为SLE组、健康体检者30例为对照组,用间接免疫荧光法检测血清抗核抗体(ANA),免疫印迹法检测可提取核抗原(ENA)多肽抗体及ds-DNA,分析各种抗体在不同患者组和对照组的阳性率.结果 在RA患者组中,ANA阳性率为37.0%,抗ds-DNA抗体阳性率为13.0%,抗ENA多肽抗体阳性率为19.6%,三种抗体联合检测阳性率为50.0%;SLE患者组中,阳性率相应分别为92.5%、70.0%、87.5%以及95.0%.结论 自身免疫性疾病患者体内存在多种自身抗体,ANA、抗ds-DNA、抗ENA多肽抗体联合检测可以相互补充,提高检出率,有利于自身免疫性疾病的诊断,避免误诊和漏诊.