山药多糖预处理对大鼠肾缺血再灌注损伤的抗氧化保护作用

摘要

目的 探讨山药多糖预处理对大鼠肾缺血再灌注损伤的保护作用及机制.方法 选择健康雄性SD大鼠30只,随机分为3组:假手术组、肾缺血再灌注损伤组、山药多糖预处理组,每组各10只,复制大鼠肾缺血再灌注损伤模型,山药多糖预处理组,于造模前1周给予200 mg/kg山药多糖灌胃.肾缺血再灌注6h后检测血清尿素氮(BUN)、血肌酐(SCr)的含量,测定肾组织超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性及丙二醛(MDA)的含量.结果 与假手术组相比,肾缺血再灌注损伤组和山药多糖预处理组血清BUN、SCr含量升高(P＜0.01),肾组织SOD、GSH-Px活性降低(P＜ 0.01),MDA含量升高(P＜0.01)；与肾缺血再灌注损伤组相比,山药多糖预处理组的血清BUN、SCr含量降低(P＜0.01),肾组织SOD、GSH-Px活性升高(P＜0.01),MDA水平降低(P＜ 0.01).结论 山药多糖预处理能改善肾缺血再灌注损伤,其机制与山药多糖抗氧化作用有关.%Objective To investigate the protective effect of Chinese yam polysaccharides pretreatment for rats with renal ischemia reperfusion injury and its mechanism.Methods 30 healthy male SD rats were randomly divided into three groups: sham operation group, ischemia reperfusion renal injury group, Chinese yam polysaccharides pretreatment group with 10 rats in each group.Then the rats renal ischemia reperfusion injury model was established.In the Chinese yam polysaccharide pretreatment group, one week befor model establishment, 200 mg/kg Chinese yam polysaccharide was given by intragastric administration.After 6 h of ischemia reperfusion, serum urea nitrogen (BUN), serum creatinine (SCr) and superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), malondialdehyde (MDA) were tested.Results Compared with sham operation group, serum levels of BUN, SCr increased (P < 0.01), the activity of SOD, GSH-Px decreased (P < 0.01) and the content of MDA increased (P < 0.01) in renal ischemia reperfusion injury group and Chinese yam polysaccharides pretreatment group.Compared with the renal ischemia reperfusion injury group, the serum levels of BUN, SCr decreased (P < 0.01), the activity of GSH-Px, SOD increased (P < 0.01), levels of MDA decreased (P < 0.01) in the Chinese yam polysaccharide pretreatment group.Conclusion Chinese yam polysae-charides pretreatment can improve renal ischemia reperfusion injury, the mechanism is related to its antioxidant effect.