高效液相色谱法测定感冒热毒清合剂中连翘苷的含量

摘要

Objective To establish a method on content determination of phillyrin in Ganmao-reduqing Mixture by high performance liquid chromatography (HPLC). Methods Phillyrin was performed qualitative identification. The content of phillyrin in mixture was determined by HPLC with a SHISEIDO CAPCELL PAK MG-C18 column (4.6 mmí250 mm, 5μm), acetonitrile-water (76:24) was used as mobile phase and the flow rate was 1.0 mL/min. The columm temperature was set at 30℃ and the wavelength of the detector was set at 277 nm. Results The linear range for phillyrin was 6.88-137.6μg/mL and the correlation cofficient was 0.9992. The average recovery of phillyrin was 99.20%, 99.96%, 98.73%respectively. Conclusion This is the first time to establish a method using HPLC for determination of phillyrin in Gan-mao-reduqing Mixture. The method is accurate, reproducible and suitable to be used for quality assessment of Gan-mao-reduqing Mixture.%目的：建立感冒热毒清合剂中连翘苷的高效液相色谱(HPLC)法含量测定方法。方法对处方中连翘进行定性鉴别，并对连翘苷进行HPLC法测定，采用色谱柱资生堂SHISEIDO CAPCELL PAK MG-C18柱(4.6 mm×250 mm，5μm)，流动相乙腈-水(76：24)，流速1.0 mL/min，柱温30℃，检测波长277 nm。结果连翘苷在6.88~137.6μg/mL范围内呈良好的线性关系(r=0.9992)，连翘苷的平均回收率为99.20%、99.96%、98.73%。结论本文首次建立了感冒热毒清合剂中连翘苷的HPLC含量测定方法，结果准确，重复性好，可作为感冒热毒清合剂的质量评价方法。