片仔癀对裸鼠人骨肉瘤肿瘤干细胞移植瘤生长的影响

摘要

Objective To explore the inhibitory effect of Pien Tze Huang on the growth of human osteosarcoma stem cells transplantation tumor of nude mice and its effect on ABCB1 and ABCG2 protein expression.Methods The model of human osteosarcoma tumor stem cell transplantation tumor was established by subcutaneous tumor stem cells in nude mice.According to the random number table method,48 nude mice were divided into control group,adriamycin group,Pien Tze Huang group,Pien Tze Huang and adriamycin combined group,with 12 mice in each group,The nude mice were executed after four weeks of intervention.The volume and weight of the tumor were measured and the tumor volume and weight inhibition rate were calculated.The expression of ABCB1 and ABCG2 in the tumor was detected by immunohistochemistry (SP) and Western blot.Results Compared with the control group,the volume and weight of tumor were significantly reduced in theadriamycin group,Pien Tze Huang group and combined group (P ＜ 0.05).Compared with Pien Tze Huang group,the combined group growth was significantly inhibited.(P ＜ 0.01).SP and Western blot results showed that the expressions of ABCB1 and ABCG2 in the Pien Tze Huang group and combined group were signif icantly down-regulated than that in the control group (P ＜ 0.05),and combined group was significantly lower than that in the Pien Tze Huang group (P ＜ 0.05).Conclusion Pien Tze Huang can significantly inhibit the growth of tumor stem cells in human osteosarcoma tumor stem cells,and it can enhance the effect of adriamycin.Which may be related to inhibit the expression of membrane transporter ABCB1 and ABCG2.%目的 探讨片仔癀对裸鼠人骨肉瘤肿瘤干细胞移植瘤的抑制作用及其对ABCB1、ABCG2蛋白表达的影响.方法 48只裸鼠皮下接种肿瘤干细胞建立人骨肉瘤肿瘤干细胞移植瘤模型.依照随机数字表法将其分为对照组、阿霉素组、片仔癀组、片仔癀与阿霉素联合组,每组各12只,分别予以相应的干预,4周后处死裸鼠,测量瘤体体积及称取其重量,计算肿瘤体积及重量抑制率;免疫组织组化学(SP法)及Western blot检测瘤体内ABCB1、ABCG2的表达.结果 与对照组比较,阿霉素组、片仔癀组、联合组瘤体体积及重量明显降低(P＜0.05),与片仔癀组比较,联合组生长受到明显抑制(P＜0.01);与对照组比较,片仔癀组、联合组可明显降低ABCB1、ABCG2的表达(P＜0.05),且联合组较片仔癀组明显下调(P＜0.05).结论 片仔癀可明显抑制人骨肉瘤肿瘤干细胞裸鼠移植瘤的生长,且能够增强阿霉素的作用,其作用机制与抑制膜转运蛋白ABCB1、ABCG2的表达相关.