葶苈生脉方中黄芪、麦冬总皂苷的提取及其含量测定

摘要

目的 研究葶苈生脉方中黄芪、麦冬皂苷的含量测定方法,并以其含量为指标优选黄芪、麦冬的最佳提取工艺.方法 选用以香草醛-冰醋酸-高氯酸为显色剂,紫外分光光度法测定总皂苷的含量.采用正交试验法,以总皂苷的含量为评价指标,考察乙醇浓度、料液比和提取时间对提取工艺的影响,优选黄芪、麦冬的最佳提取工艺.结果 本研究中黄芪甲苷在8.88～44.40 μg范围内呈良好的线性关系,精密度、稳定性、重现性试验的RSD值均≤1.19％,平均加样回收率为101.78％,RSD值为1.85％(n=9).最佳提取工艺:8倍量80％的乙醇回流提取2次,每次2h.结论 该方法操作简便,准确、重现性好,可作为葶苈生脉方中黄芪、麦冬皂苷质量控制的方法.%Objective To study the content determination method of total saponins from Astragali Radix and Ophiopogonis Radix in Tingli Shengmai Decoction,and to optimize the optimum extraction process of Astragali Radix and Ophiopogonis Radix taking the contents as indices.Methods The vanillic aldehyde-glacial acetic acid-perchloric acid was selected as color developing agent,and the contents of total saponins were detected by ultraviolet spectrophotometry.By using orthogonal experiment,taking the contents of total saponins as indices,the effects of ethanol concentration,solid-liquid ratio and extraction duration for the extraction process were investigated,so as to optimize the optimum extraction process of Astragali Radix and Ophiopogonis Radix.Results In this study,astragaloside Ⅳ showed a good linear relationship within the range of 8.88-44.40 μg,all the RSD values of degree of precision,stability and repeatability experiments were ≤ 1.19％,the average recovery rate was 101.78％,RSD was 1.85％ (n =9).The optimum extraction process:reflux extraction of 8 times amount of 80％ ethanol was taken for 2 times,once 2 h.Conclusion The method is simple,accurate and reproducible,which can be used as a method for the quality control of Astragali Radix and Ophiopogonis Radix in Tingli Shengmai Decoction