Content Determination of Total Saponins in Different Parts of Plant Paris Polyphylla Var. Chinensis

摘要

Purpose: Test the content determination of total saponins in different parts of the plant Paris Polyphylla Var. Chinensis. Method: Take dioscin as reference substance and adopts ultraviolet spectrophotometry on the place of 310.5 nm to test the content determination of total saponins on different parts of the plant Paris Polyphylla Var. Chinensis. Results: the linearity of dioscin in the scope of 0.128mg~0.768mg is good, and the regression equation is A=3.1775C+0.2668 (r=0.9994); the test results of average recovery shows that the average recovery ratio is 101.13%, and RSD is 2.31 %(n=6). Conclusion: there are larger differences in the content determination of total saponins on different parts of the plant Paris Polyphylla Var. Chinensis; among them, the content in the rhizome of Paris Polyphylla Var. Chinensis is the highest - 2.021%, the secondary high content is in the root - 0.263%, while dioscin is not detected in the terrestrial stem and leaves.