基于生物介质的丙三醇对分子荧光强度影响的研究

摘要

目的:生物介质中加入丙三醇是一种保存荧光样品的重要方法,研究丙三醇含量对生物介质中不同发射波长荧光分子的荧光强度的影响.方法:以磷酸盐缓冲液(PBS)作为介质,测定不同丙三醇体积分数下的拓扑替康、磺酰罗丹明B(SRB)、吖啶橙(AO)和罗丹明B的荧光强度以及丙三醇的荧光光谱和紫外-可见吸收光谱.结果:研究发现丙三醇本身无荧光,但其能够增强上述荧光分子的荧光强度.随着丙三醇体积分数的增大,荧光分子的荧光强度逐渐增强.结论:生物介质中加入丙三醇后能够增强分子的荧光强度.选择含高体积分数丙三醇的生物介质,有利于提高荧光分子检测的灵敏度.%Methods: The study was designed to determine the changes of the fluorescent intensity of topotecan, Sulforhodamine B, Acridine orange and Rhodamine B in phosphate buffer saline solution with different concentration of glycerol, and the fluorescence and ultraviolet-visible absorption spectra of glycerol. Results: The results showed that glycerol itself has no fluorescence but can affect fluorescent intensity of the fluorescent molecules, Furthermore, the higher the concentration of glycerol was, the stronger the fluorescent intensity of molecules was. Conclusions: Adding glycerol to biological medium can strengthen the fluorescent intensity of molecules. Selecting high percentage of glycerol solution is an approach to improve the detection sensitivity of the above fluorescent molecules.