股骨干骨折合并脑损伤后大鼠多器官功能损害的生化指标观察

摘要

目的:对大鼠血清中多种生化指标的检测.探讨股骨干骨折合并脑损伤后对大鼠多器官功能的影响.方法:4月龄雄性SD大鼠30只,体重(280±10)g,用随机数字表法将大鼠随机分为对照组,创伤第1天组,创伤第2天组,创伤第3天组,创伤第5天组,创伤第7天组,每组5只.5个创伤组制造大鼠右侧股骨干骨折合并脑损伤的模型.然后分别在造模后第1,2,3,5,7天抽取各组腹主动脉,检测血清中天冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)、肌酐(Cr)、尿素氮(BUN)、乳酸脱氢酶(LDH)、磷酸肌酸激酶(CK)的含量,对照组不做任何处理.只进行上述检测.对各组检测的生化指标进行比较.结果:各组血清中AST、ALT、Cr、BUN、LDH、CK含量差异均有统计学意义(P<0.01),其中AST、ALT、BUN、CK在创伤第1天组达到峰值(P<0.05);Cr在创伤第3天组达到峰值(P<0.05);LDH在创伤第2天组达到峰值(P<0.05).结论:股骨干骨折合并脑损伤后会引起大鼠血清中多种生化指标的升高,尤其以创伤后前3 d的影响较为显著,这些生化指标改变可间接的反应心、肝、肾器官的功能下降.%Objective :To explore the features of rats organs functional changes after femur shaft fracture combined with brain injury through testing biochemical indicators in rats.Methods:Thirty 4-month-old male SD rats,weight(280±10) g,were divided into 5 groups according to random number table involving normal control group, at the 1st day of injured group, the 2nd day injured group,the 3rd day injured group,the 5th day injured group,the 7th day injured group,5 rats in each group.The animal injury model of right femur shaft fracture combined with brain injury were made by instruments in 5 injured groups.At the 1st,2nd,3rd,5th,7th day after made model,the biochemical indicators of blood serum from abdominal aorta including AST, ALT, Cr, BUN, LDH and CK were detected and compared.Results :The biochemical indicator of blood serum (AST,ALT,Cr,BUN ,LDH,CK) changed significantly among different groups.AST ,ALT,BUN and CK reached peaks at the 1st day after injured (P＜0.05).Cr reached peaks at the 3rd day after injured (P＜0.05).LDH reached peak at the 2nd day after injured (P＜0.05).Conclusion:After brain injury combined with right femoral fractures in rats, a variety of serum biochemical indicators increase significantly,especially in early 3 days after injured.These indicators shows indirectly heart,liver and kidney organ dysfunction by trauma.