丙泊酚对大鼠脊髓星形胶质细胞释放兴奋性氨基酸的影响

摘要

[Objective]To investigate the influence of propofol on excitory amino acids (EAA) release of astrocytes in the spinal cord of rats activated by tumor necrosis factor-α (TNF-α). [Methods] The purified cultured astrocytes in the spinal cord of rats were divided into 4 groups: containing 1μg/L TNF-α and 25 μmol/L propofol (group TP), containing 1 μg/L TNF-α (group T), lipid vehicle (Intralipid, 0.2 ml/L, group L), control group (group C).By means of high performance liquid chromatography (HPLC), the contents of Glutamic acid (Glu) and Aspartic acid (Asp) in the supernatants of astrocytes were measured at 0, 30, 60and 120 min. [Results] The contents of Glu and Asp in group L were not different from those in group C (P ＞0.05). The contents of Glu and Asp increased as time went on in groupT and group TP. The contents of Glu and Asp in group TP were evidently lower than those in group T at 60, 120 min (P ＜0.05). [Conclusion] The release of excitory amino acides stimulated by tumor necrosis factorα was is inhibited by propofol.%目的 研究被肿瘤坏死因子(TNF-α)刺激的大鼠脊髓星形胶质细胞(AST)在丙泊酚作用下兴奋性氨基酸(EAA)的释放情况.方法 体外纯化培养大鼠脊髓星形胶质细胞,分为TNF-α终浓度为1μg/L及丙泊酚终浓度为25μmol/L(TP组),TNF-α终浓度为1μg/L(T组),乳剂对照组(Intralipid,0.2ml/L、L组),空白对照组(C组).用高效液相色谱仪测定各组培养细胞在0、30、60、120 min时谷氨酸(Glu),天门冬氨酸(Asp)的释放量.结果 与C组相比,L组的Glu、Asp释放量无明显差异(P＞0.05);T、TP组Glu、Asp释放量随着时间的延长而增加;与T组相比,60、120 min时,TP组Glu、Asp释放量明显减少(P＜0.05).结论 TNF-α刺激AST释放EAA,而丙泊酚对此有抑制作用.