丁酸钠对严重烫伤后大鼠肝脏血流量及水肿的影响

摘要

目的：研究丁酸钠对严重烫伤后大鼠肝脏血流量及水肿的影响。方法雄性SD大鼠48只，体重（250±10）g，随机分为正常组、烫伤+生理盐水组（对照组）和烫伤+丁酸钠组（丁酸钠组）。对照组和丁酸钠组采用沸水烫伤背部15 s、腹部8 s，造成50%总体表面积（TBSA）Ⅲ度烫伤，烫伤后立即腹腔注射丁酸钠（400 mg/kg）或等体积生理盐水治疗，正常组腹腔注射丁酸钠（400 mg/kg）。烫伤后3和6 h采用激光多普勒血流仪测定肝脏血流量；心脏穿刺取血检测谷丙转氨酶（ALT）；取肝脏组织检测组织含水率及肝脏微血管通透性。结果烫伤后3和6 h，与正常组比较，对照组肝脏血流量均显著减少，微血管通透性、含水率、血浆ALT均明显升高；烫伤后3和6 h，与对照组比较，丁酸钠组微血管通透性、肝脏含水率、血浆ALT均明显降低，血流量均显著增加。结论丁酸钠减轻严重烫伤后肝脏水肿，增加肝脏血流量，对肝脏功能具有一定的保护作用。%Objective To investigate the effects of sodium butyrate (BTR) on hepatic blood flow (HBF) and tissue edema in rats following severe empyrosis. Methods Forty-eight male SD rats, weighting (250±10) g, were randomly divided into three groups: normal+BTR (NB) group, empyrosis+normal saline (EN) group and empyrosis +BTR (EB) group. The rats in the EN and EB groups were subjected to 50% TBSA third-degree burns by immersing the back in boiling water for 15 seconds and the abdomen for 8 seconds, and then BTR (400 mg/kg) or normal saline was intraperitoneally injected, respectively. The NB group was intraperitoneally injected with BTR (400 mg/kg). Hepatic blood flow was measured by laser Doppler 3 and 6 hours after injury. Meanwhile, blood was drawn from the heart for measurement of plasma alanine aminotransferase (ALT). The tissue samples of liver were harvested for the measurement of microvascular permeability and tissue water content. Results The plasma level of ALT, the tissue water content and the microvascular permeability of liver significantly increased while the HBF level significantly decreased in the EN group compared with the NB group at 3 and 6 hours after injury. Compared with the EN group, the plasma level of ALT, the microvascu-lar permeability and the tissue water content in the EB group were significantly lowered at 3 and 6 hours af ter empyrosis. In addition, BTR treatment significantly increased the HBF level following empyrosis both at 3 and 6 hours after empyrosis. Conclusions The results indicated that sodium butyrate has significantly pro-tective effects on liver in rats with severe empyrosis by alleviating hepatic edema and increasing blood flow to liver.